Als het "Museum of Natural History" gesloten wordt vanwege renovaties, moeten de museumstukken overgebracht worden naar het beroemde "Washington Museum". Larry Daley (Ben Stiller) komt terecht in de zogenaamde Smithsonian-instelling van het museum, waar hij de opdracht krijgt om een aantal museumstukken te redden, omdat deze verkeerd zijn verscheept. Eenmaal geïnfiltreerd in het Smithsonian ziet Larry dat er weer leven zit in alle museumstukken, waaronder Amelia Earhart (Amy Adams), Al Capone en vele andere historische personen.
Nee, helaas is Night at the Museum: Battle of the Smithsonian nog niet beschikbaar op Netflix in Nederland.
| Directing | Shawn Levy | Director |
| Production | Michael Barnathan | Producer |
| Writing | Thomas Lennon | Screenplay |
| Production | Chris Columbus | Producer |
| Production | Shawn Levy | Producer |
| Lighting | Aravind Jayaraman | Lighting Artist |
| Visual Effects | Guy Bar'ely | Animation |
| Visual Effects | Joel Foster | Animation |
| Visual Effects | Julie D'Antoni | Visual Effects |
| Crew | Sagar Patil | Visual Effects Art Director |
| Crew | Mike Marcuzzi | Digital Effects Supervisor |
| Visual Effects | Daniel Lindsey | VFX Artist |
| Crew | James Bamford | Stunts |
| Crew | J.J. Makaro | Stunt Coordinator |
| Art | Helen Jarvis | Supervising Art Director |
| Camera | John Schwartzman | Director of Photography |
| Sound | Alan Silvestri | Music |
| Crew | Greg Hanson | Stunt Double |
| Crew | Bill Anagnos | Utility Stunts |
| Crew | Loyd Bateman | Stunts |
| Crew | Fraser Aitcheson | Stunts |
| Crew | Chad Bellamy | Stunts |
| Crew | Nickolas Baric | Stunts |
| Crew | Robert Alonzo | Utility Stunts |
| Crew | Krista Bell | Stunt Double |
| Crew | Trevor Addie | Stunts |
| Crew | Lloyd Adams | Stunts |
| Production | Donna Isaacson | Casting |
| Costume & Make-Up | Marlene Stewart | Costume Design |
| Visual Effects | Dan DeLeeuw | Visual Effects Supervisor |
| Production | Ellen Somers | Associate Producer |
| Editing | Don Zimmerman | Editor |
| Editing | Dean Zimmerman | Editor |
| Art | Claude Paré | Production Design |
| Production | Thomas M. Hammel | Executive Producer |
| Production | Josh McLaglen | Executive Producer |
| Production | Mark Radcliffe | Executive Producer |
| Production | Anne M. Simonet | Unit Production Manager |
| Production | Thomas M. Hammel | Unit Production Manager |
| Directing | Josh McLaglen | First Assistant Director |
| Directing | Misha Bukowski | Second Assistant Director |
| Production | Eric Hedayat | Production Supervisor |
| Crew | Garrett Warren | Stunt Coordinator |
| Directing | Gary Capo | Second Unit Director |
| Art | Lin MacDonald | Set Decoration |
| Crew | William H. Orr | Special Effects Coordinator |
| Art | Dean Eilertson | Property Master |
| Art | Jason B. Landels | Assistant Property Master |
| Art | Michelle Hendriksen | Assistant Property Master |
| Art | Claudio Palavecino | Assistant Property Master |
| Art | Grant Van Der Slagt | Art Direction |
| Art | Michael Diner | Art Direction |
| Art | Sean Goojha | Art Department Coordinator |
| Crew | Lance White | Video Assist Operator |
| Camera | Ian Fox | "A" Camera Operator |
| Camera | Ian Fox | Second Unit Director of Photography |
| Camera | David Crone | "B" Camera Operator |
| Camera | David Crone | Steadicam Operator |
| Camera | Richard Mosier | First Assistant "A" Camera |
| Camera | Patrick Stepien | First Assistant "B" Camera |
| Camera | Justin Bergler | Second Assistant Camera |
| Camera | Tyler Woeste | Second Assistant Camera |
| Camera | Jean-Marc Saldini | Camera Loader |
| Camera | Doane Gregory | Still Photographer |
| Sound | Rob Young | Production Sound Mixer |
| Sound | Kelly Zombor | Boom Operator |
| Sound | Darryl Marko | Utility Sound |
| Directing | Diane Durant | Script Supervisor |
| Editing | Herbert Dwight Raymond IV | First Assistant Editor |
| Crew | Jeff Beattie | Visual Effects Editor |
| Sound | Terry Wilson | Supervising Music Editor |
| Sound | Craig Henighan | Supervising Sound Editor |
| Sound | John A. Larsen | Supervising Sound Editor |
| Sound | Paul Massey | Sound Re-Recording Mixer |
| Sound | David Giammarco | Sound Re-Recording Mixer |
| Art | Laurie Dobbie | Property Buyer |
| Art | Michael A. Billings | Assistant Set Decoration |
| Art | Zoe Jirik | Set Decorating Coordinator |
| Art | Joel Venti | Storyboard Artist |
| Art | Michael Anthony Jackson | Storyboard Artist |
| Art | Marc Baird | Storyboard Artist |
| Art | Mira Caveno | Set Designer |
| Art | Jay Mitchell | Set Designer |
| Art | Bryan Sutton | Set Designer |
| Production | Barbara Harris | ADR Voice Casting |
| Costume & Make-Up | Victoria Down | Makeup Department Head |
| Costume & Make-Up | Sherry Linder-Gygli | Hair Department Head |
| Lighting | Andrew W. Davidson | Gaffer |
| Lighting | Sean Oxenbury | Rigging Gaffer |
| Lighting | Keith Woods | Rigging Gaffer |
| Lighting | Shane Dobie | Best Boy Electric |
| Camera | Mike Kirilenko | Key Grip |
| Lighting | David McIntosh | Key Rigging Grip |
| Camera | David Cameron | Best Boy Grip |
| Camera | Ryan Monro | Dolly Grip |
| Camera | Darin Wong | Dolly Grip |
| Costume & Make-Up | John Casey | Costume Supervisor |
| Costume & Make-Up | Jana MacDonald | Costume Coordinator |
| Production | John Catron | Production Accountant |
| Production | Fawn McDonald | Production Coordinator |
| Production | Rino Pace | Location Manager |
| Production | Jason Collier | Assistant Location Manager |
| Directing | Daniel Mansfield | Second Second Assistant Director |
| Art | John Dale | Construction Coordinator |
| Art | Mario Tomas-Niedworok | Paint Coordinator |
| Crew | Patricia Johnson | Unit Publicist |
| Crew | Rob Vreugde | Transportation Coordinator |
| Sound | Craig Henighan | Sound Designer |
| Sound | R.J. Kizer | Supervising ADR Editor |
| Sound | Laura Graham | ADR Editor |
| Sound | Vanessa Lapato | ADR Editor |
| Sound | Coll Anderson | Sound Effects Editor |
| Sound | John Murray | Foley Supervisor |
| Sound | Scott Curtis | Foley Editor |
| Sound | Doug Jackson | Foley Editor |
| Sound | Charleen Richards-Steeves | ADR Mixer |
| Sound | Skip Longfellow | First Assistant Sound Editor |
| Sound | Jason Tuttle | Assistant Sound Editor |
| Sound | Eric A. Larsen | Assistant Sound Editor |
| Sound | Tim Gomillion | Sound Recordist |
| Sound | David Lucarelli | ADR Recordist |
| Sound | Dan O'Connell | Foley Artist |
| Sound | John T. Cucci | Foley Artist |
| Sound | James Ashwill | Foley Mixer |
| Editing | Jim Passon | Color Timer |
| Editing | Eileen Godoy | Digital Intermediate Producer |
| Editing | Devon Miller | Digital Intermediate Editor |
| Visual Effects | Raymond Chen | Visual Effects Supervisor |
| Visual Effects | Bill Tlusty | Visual Effects Producer |
| Art | Audra Neil | Set Decoration Buyer |
| Writing | Milan Trenc | Characters |
| Writing | Robert Ben Garant | Screenplay |
| Crew | Jennifer Mylrea | Utility Stunts |
| Crew | Bianca Giancoli | Utility Stunts |
| Crew | Kimberly Chiang | Stunts |
| Crew | J.J. Perry | Utility Stunts |
| Crew | Lauro David Chartrand-DelValle | Stunts |
| Crew | Clint Carleton | Stunt Double |
