Na door een genetisch gemanipuleerde spin gebeten te zijn, maakt het lichaam van de nerd Peter Parker radicale wijzigingen door. Hij kan tegen muren oplopen, web-vloeistof uit zijn polsen spuiten, en hij ontwikkelt een spinnen-zintuig. Hij neemt de naam "Spider-Man" aan, en besluit zijn nieuwe krachten te gebruiken om de slechte Green Goblin te bestrijden. Ondertussen probeert hij de aandacht te trekken van zijn mooie klasgenote Mary Jane Watson.
| Costume & Make-Up | James Acheson | Costume Design |
| Camera | Don Burgess | Director of Photography |
| Art | Neil Spisak | Production Design |
| Editing | Bob Murawski | Editor |
| Art | Karen O'Hara | Set Decoration |
| Production | Laura Ziskin | Producer |
| Art | Tony Fanning | Art Direction |
| Production | Ian Bryce | Producer |
| Sound | Danny Elfman | Original Music Composer |
| Editing | Arthur Coburn | Editor |
| Production | Avi Arad | Executive Producer |
| Writing | David Koepp | Screenplay |
| Production | Francine Maisler | Casting |
| Art | Stella Vaccaro | Art Direction |
| Visual Effects | Alyssa Fong | Visual Effects |
| Production | Heidi Fugeman | Associate Producer |
| Production | Grant Curtis | Co-Producer |
| Production | Steven P. Saeta | Associate Producer |
| Production | Lynn Kressel | Casting |
| Costume & Make-Up | Deborah La Mia Denaver | Makeup Department Head |
| Camera | Bruce Del Castillo | Grip |
| Art | Steve Arnold | Supervising Art Director |
| Directing | Jeff Shiffman | Assistant Director |
| Art | Scott P. Murphy | Art Direction |
| Costume & Make-Up | Janice Alexander | Hair Department Head |
| Crew | Richard L. Blackwell | Stunts |
| Visual Effects | Blondel Aidoo | Visual Effects Producer |
| Crew | Tony Ching Siu-Tung | Stunt Coordinator |
| Sound | Dean Beville | Sound Editor |
| Visual Effects | Jacquie Barnbrook | Visual Effects Producer |
| Sound | Peter Brown | Sound Effects Editor |
| Crew | George P. Wilbur | Stunts |
| Camera | Al Cerullo | Helicopter Camera |
| Crew | Steve Riley | Special Effects |
| Camera | Tony Arnaud | Grip |
| Camera | Zade Rosenthal | Still Photographer |
| Art | Jon J. Bush | Leadman |
| Costume & Make-Up | Martha F. Baxley | Costume Supervisor |
| Crew | Amin Chande | Craft Service |
| Sound | Ed Novick | Sound Mixer |
| Visual Effects | John Frazier | Special Effects Supervisor |
| Costume & Make-Up | Carol 'Ci Ci' Campbell | Hairstylist |
| Crew | Michael Wilson | Software Engineer |
| Editing | Patrick Gallagher | First Assistant Editor |
| Sound | Zigmund Gron | Music Editor |
| Art | François Audouy | Assistant Art Director |
| Art | Amy Safhay | Greensman |
| Crew | J.C. Brotherhood | Special Effects Coordinator |
| Crew | Christopher D. Childers | Choreographer |
| Crew | Guy Belegaud | Propmaker |
| Lighting | John L. Oates | Gaffer |
| Costume & Make-Up | Linda Grimes | Makeup Artist |
| Crew | Mitch Goldstrom | Systems Administrators & Support |
| Production | Denise Morgan McGrath | Production Accountant |
| Costume & Make-Up | Lisa Tomczeszyn | Assistant Costume Designer |
| Crew | Daniel Eaton | CG Supervisor |
| Crew | Willow Craven | Post Production Assistant |
| Crew | Heather Wusterbarth | Set Production Assistant |
| Crew | Robyne Parrish | Stand In |
| Lighting | Erik Bernstein | Best Boy Electric |
| Crew | Sandy O'Neill | Unit Publicist |
| Editing | Jim Passon | Color Timer |
| Visual Effects | Karen E. Goulekas | Visual Effects Supervisor |
| Art | Mick Cukurs | Set Designer |
| Lighting | Larry J. Aube | Rigging Grip |
| Visual Effects | Lloyd Lee Barnett | Digital Compositors |
| Art | Miles Teves | Conceptual Design |
| Costume & Make-Up | Alec Gillis | Makeup Designer |
| Crew | Robin L. Miller | Property Master |
| Crew | Kevin Abercrombie | Utility Stunts |
| Production | Candice D. Campos | Production Coordinator |
| Crew | James Marbas | Digital Effects Supervisor |
| Crew | Jeff Habberstad | Stunt Coordinator |
| Crew | Edward Fanning | Transportation Co-Captain |
| Sound | Steve Bartek | Orchestrator |
| Camera | Thane Berti | Camera Operator |
| Crew | Derrick Alford | Carpenter |
| Crew | Mike Manzel | Makeup Effects |
| Visual Effects | Dennis Webb | I/O Supervisor |
| Art | Ilana Gordon | Art Department Assistant |
| Art | Daren Cornell | Painter |
| Camera | James Bartolomeo | First Assistant Camera |
| Crew | Scott 'Ice Paq' Baron | Set Medic |
| Lighting | James Barrett | Electrician |
| Production | Kathy Driscoll-Mohler | Casting Associate |
| Art | Ozzy Inguanzo | Art Department Coordinator |
| Art | Mike Larrabee | Lead Painter |
| Crew | Randy Peters | Transportation Captain |
| Art | Kathleen Beall | Location Scout |
| Art | Harald Belker | Production Illustrator |
| Art | Robert E. Denne | Standby Painter |
| Crew | Kevin J. Jolly | Visual Effects Editor |
| Directing | Susana Preston | Script Supervisor |
| Art | Gene 'Hap' Cooper | Sculptor |
| Crew | Michael Avallon | Driver |
| Crew | Ann Marie Auricchio | Scenic Artist |
| Crew | Michael J. Hogan | Video Assist Operator |
| Production | Susan Dukow | Production Supervisor |
| Sound | Christopher O'Donnell | Boom Operator |
| Sound | Susan Dudeck | Sound Designer |
| Visual Effects | Victor DiMichina | Visual Effects Coordinator |
| Art | Jim Ondrejko | Construction Coordinator |
| Camera | Dan Moore | Additional Photography |
| Costume & Make-Up | Sonya M. Andonov | Set Costumer |
| Production | Richard Baratta | Unit Production Manager |
| Lighting | Anthony D. Guzman | Lighting Technician |
| Production | John Fedynich | Location Manager |
| Production | Steven P. Saeta | Production Manager |
| Lighting | Kevin Murphy | Chief Lighting Technician |
| Editing | Greg Socher | Associate Editor |
| Sound | Tom Boyd | Musician |
| Sound | Brad Kanfer | Apprentice Sound Editor |
| Art | Michael Gastaldo | Assistant Property Master |
| Camera | Michael J. Coo | Key Grip |
| Visual Effects | Bill Diaz | Lead Animator |
| Crew | Carey Smith | Digital Effects Producer |
| Writing | Steve Montal | Story Editor |
| Crew | Chuck Jeffreys | Fight Choreographer |
| Sound | Alison Fisher | ADR Editor |
| Visual Effects | Bernie Wrightson | Character Designer |
| Costume & Make-Up | Michele Flynn | Ager/Dyer |
| Editing | Mo Henry | Negative Cutter |
| Production | Nancy Safran | Casting Assistant |
| Art | Ahmet Ahmet | Title Designer |
| Costume & Make-Up | Cynthia Black | Key Costumer |
| Camera | Michael Brennan | Dolly Grip |
| Directing | Michael J. Moore | Second Assistant Director |
| Camera | Steven Cueva | Camera Loader |
| Sound | Dana Gustafson | Foley Editor |
| Art | Tristan Paris Bourne | Set Dresser |
| Costume & Make-Up | Bill Myer | Key Makeup Artist |
| Directing | Eric Heffron | First Assistant Director |
| Sound | Jamie Hardt | Assistant Sound Editor |
| Visual Effects | Colin Drobnis | Visual Effects Technical Director |
| Production | Jenifer Bonisteel | Assistant Production Coordinator |
| Visual Effects | John Dykstra | Visual Effects Designer |
| Crew | Eric Hayden | Special Effects Technician |
| Art | James Carson | Concept Artist |
| Sound | Peter Gleaves | ADR Mixer |
| Sound | Karen Glauber | Music Consultant |
| Crew | Chris Walden | Generator Operator |
| Production | Shannon Gaulding | Production Executive |
| Crew | Ty Williams | Stunt Driver |
| Visual Effects | Pedram Goshtasbpour | Senior Animator |
| Production | Alex Borys | Location Assistant |
| Production | Greg Outcalt | Production Secretary |
| Costume & Make-Up | David Butler | Costumer |
| Camera | Joseph Aspromonti | Additional Second Assistant Camera |
| Production | Linda Weaver | Extras Casting Assistant |
| Production | Rob Dickerson Jr. | Key Production Assistant |
| Directing | John Elmore | Second Second Assistant Director |
| Production | Ray Scalice | General Manager |
| Costume & Make-Up | Jennifer Saeta | Assistant Makeup Artist |
| Sound | Brian Ruberg | Foley Mixer |
| Visual Effects | Chris Antonini | Visual Effects Production Assistant |
| Camera | Kent Alexander | Dolly Grip |
| Production | Chris Bustard | Additional Casting |
| Editing | Steve Bowen | Digital Colorist |
| Production | Michael Wesley Aycock | Assistant Location Manager |
| Sound | Matthew Dettmann | Foley Artist |
| Camera | Herb Ault | Additional Key Grip |
| Editing | Anthony Harris | Digital Color Timer |
| Camera | Kelly Diehl | Libra Head Technician |
| Editing | Jody Fedele | Assistant Editor |
| Production | Alexa Song Lindenthaler | Second Assistant Accountant |
| Crew | Chris Waegner | Technical Advisor |
| Production | Gavin J. Behrman | First Assistant Accountant |
| Production | Geoffrey Casquias | Production Assistant |
| Crew | Dion Hatch | Digital Supervisor |
| Art | Mark Andrews | Storyboard Artist |
| Camera | Richard E. Garcia | Assistant Camera |
| Sound | Pete Anthony | Conductor |
| Lighting | Alan Frazier | Assistant Chief Lighting Technician |
| Art | Michael Marcus | Art Designer |
| Art | Jonathan Graham | Key Construction Grip |
| Production | Kelley Baker | Assistant Accountant |
| Crew | Darrell Burgess | Special Effects Technician |
| Camera | Craig Kohtala | Best Boy Grip |
| Production | Shawn P. McKeegan | Art Department Production Assistant |
| Art | Dianne Chadwick | Graphic Designer |
| Art | Joanna Venezky | Set Buyer |
| Camera | Cheli Clayton | Second Assistant Camera |
| Visual Effects | Anthony B. LaMolinara | Animation Director |
| Production | Michael Anthony Scott | Additional Production Assistant |
| Directing | Doug Lefler | Second Unit Director |
| Crew | Robin Torell | Stunt Double |
| Production | Dave McComb | Accountant |
| Production | Chad Darnell | Extras Casting |
| Production | Joan Devilla | Payroll Accountant |
| Costume & Make-Up | Helen Wilson | Seamstress |
| Sound | Sean Landeros | Sound Recordist |
| Visual Effects | Scott Stokdyk | Visual Effects Supervisor |
| Visual Effects | John Dykstra | Visual Effects Supervisor |
| Visual Effects | Ray McIntyre Jr. | Visual Effects Supervisor |
| Sound | Greg P. Russell | Sound Re-Recording Mixer |
| Sound | Kevin O'Connell | Sound Re-Recording Mixer |
| Sound | David A. Arnold | Dialogue Editor |
| Directing | Sam Raimi | Director |
| Crew | Mike Smith | Stunts |
| Production | Stan Lee | Executive Producer |
| Writing | Stan Lee | Comic Book |
| Writing | Steve Ditko | Comic Book |
| Sound | Stephen Hunter Flick | Sound Designer |
| Costume & Make-Up | Gary Archer | Prosthetics |
| Costume & Make-Up | Wendi Lynn Avignone | Makeup Artist |
| Crew | Spice Williams-Crosby | Stunts |
| Crew | Nancy Thurston | Stunts |
| Crew | Annie Ellis | Stunt Double |
| Crew | Rick Avery | Stunts |
| Crew | Joni Avery | Stunts |
| Production | Bill Hader | Production Assistant |
| Crew | Mark De Alessandro | Stunts |
| Crew | Laura Albert | Stunts |
| Crew | Dane Farwell | Stunts |
| Crew | Paul E. Short | Stunts |
| Crew | Al Goto | Stunts |
| Production | Kevin Feige | Production Executive |
| Crew | Lisa Hoyle | Stunts |
