De Professor en Luke staan voor een nieuw raadsel als de jonge zangeres Janice Quatlane hen vertelt dat ze de geest van haar overleden vriendin heeft gezien. Een heer als Layton is kan deze jongedame in haar verwarring niet in de steek laten. Om het mysterie op te lossen gaan Luke en de Professor naar het wereldberoemde operahuis 'Crown Petone'. Daar gaat de laatste opera van de geniale componist Oswald Whistler in première. Daar, blijkt de voorstelling uit te lopen op een angstaanjagend spel: de winnaar maakt kans om onsterfelijk te worden! De sleutel tot dit raadsel ligt blijkbaar in het eeuwige leven.
Nee, helaas is Professor Layton en de Eeuwige Diva nog niet beschikbaar op Netflix in Nederland.
| Directing | 橋本昌和 | Director |
| Sound | 西浦智仁 | Original Music Composer |
| Visual Effects | 佐藤孝一 | Key Animation |
| Visual Effects | 徳田夢之介 | Key Animation |
| Visual Effects | 小倉陳利 | Key Animation |
| Visual Effects | 堀内博之 | Key Animation |
| Visual Effects | 清水洋 | Key Animation |
| Visual Effects | 岡村玲子 | Key Animation |
| Visual Effects | 杉光登 | Key Animation |
| Visual Effects | 久保光寿 | Key Animation |
| Visual Effects | 海谷敏久 | Key Animation |
| Visual Effects | 杉光登 | Animation Director |
| Visual Effects | 堀内博之 | Animation Director |
| Visual Effects | 松原徳弘 | Animation Director |
| Visual Effects | 一石小百合 | Animation Director |
| Visual Effects | 吉野真一 | Animation Director |
| Visual Effects | 鍋田香代子 | Animation Director |
| Visual Effects | Hideki Takahashi | Animation Director |
| Visual Effects | 川面恒介 | Animation Director |
| Visual Effects | 川面恒介 | Key Animation |
| Visual Effects | 鍋田香代子 | Key Animation |
| Visual Effects | 菅沼芙実彦 | Key Animation |
| Visual Effects | 早川麻美 | Key Animation |
| Visual Effects | 藤井康雄 | Key Animation |
| Visual Effects | 小島明日香 | Key Animation |
| Visual Effects | 戸谷賢都 | Key Animation |
| Visual Effects | 大東百合恵 | Key Animation |
| Visual Effects | 大導寺美穂 | Key Animation |
| Visual Effects | Katsumi Ikeda | Key Animation |
| Visual Effects | 牧孝雄 | Key Animation |
| Visual Effects | 吉岡毅 | Key Animation |
| Visual Effects | 石井明治 | Key Animation |
| Visual Effects | 入江健司 | Key Animation |
| Visual Effects | 上田美由紀 | Key Animation |
| Visual Effects | 小島彰 | Key Animation |
| Visual Effects | 伊勢奈央子 | Key Animation |
| Visual Effects | 中西彩 | Key Animation |
| Visual Effects | 津田崇 | Key Animation |
| Visual Effects | 緒方厚 | Key Animation |
| Visual Effects | 大野泰江 | Key Animation |
| Visual Effects | 小山知洋 | Key Animation |
| Visual Effects | 小川智樹 | Key Animation |
| Visual Effects | Junpei Fukuchi | Key Animation |
| Visual Effects | Hosato Shima | Key Animation |
| Visual Effects | 鈴木美咲 | Key Animation |
| Visual Effects | 牧野博美 | Key Animation |
| Visual Effects | 東海林康和 | Key Animation |
| Visual Effects | 深沢幸司 | Key Animation |
| Visual Effects | 尾鷲英俊 | Key Animation |
| Visual Effects | 宮田忠明 | Key Animation |
| Visual Effects | 吉田優子 | Key Animation |
| Visual Effects | 宮岡真弓 | Key Animation |
| Visual Effects | 夏住愛子 | Key Animation |
| Visual Effects | 岩岡優子 | Key Animation |
| Visual Effects | 黒石崇裕 | Key Animation |
| Production | 久保雅一 | Executive Producer |
| Production | 濱名一哉 | Executive Producer |
| Production | 藤巻直哉 | Co-Executive Producer |
| Production | 阿部秀司 | Co-Executive Producer |
| Art | 橋本昌和 | Storyboard Artist |
| Art | 今石洋之 | Storyboard Artist |
| Art | 志村錠児 | Storyboard Artist |
| Art | 西村純二 | Storyboard Artist |
| Art | 増井壮一 | Storyboard Artist |
| Sound | 山谷尚人 | Sound Effects |
| Sound | 西佐知子 | Sound Effects |
| Sound | 緒方康恭 | Sound Effects |
| Sound | 三井友和 | Sound Effects |
| Sound | 長谷川卓也 | Sound Effects |
| Sound | 武藤晶子 | Sound Effects |
| Sound | 倉橋静男 | Sound Effects |
| Sound | 三間雅文 | Sound Director |
| Art | 竹田悠介 | Art Direction |
| Editing | 辺見俊夫 | Editor |
| Art | 篠原理子 | Art Direction |
| Visual Effects | 杉光登 | Character Designer |
| Visual Effects | 杉光登 | Supervising Animation Director |
| Writing | 松井亜弥 | Screenplay |
| Production | 奥野敏聡 | Producer |
| Production | 堀川憲司 | Producer |
| Production | 岡田有正 | Producer |
| Production | 山内章弘 | Producer |
| Sound | 斉藤恒芳 | Main Title Theme Composer |
| Production | 阿蘇博 | Co-Producer |
| Production | Toshihiro Kitta | Co-Producer |
| Production | Koji Goromaru | Co-Producer |
| Production | 菊池宣広 | Co-Producer |
| Production | 細谷まどか | Co-Producer |
| Art | 和田典子 | Background Designer |