Larry Daley gelooft, ondanks zijn tot op heden ongelukkige leven, dat er iets groots voor hem staat te gebeuren. Dat blijkt wel als hij aangenomen wordt als nachtwaker bij het "Museum of Natural History". Tijdens zijn diensten beginnen er vreemde dingen te gebeuren. Gladiatoren en cowboys voeren een nachtelijke strijd terwijl een Neanderthaler zijn eigen display verbrandt, en de T-rex iedereen laat zien waarom hij het meest gevreesde roofdier is uit de geschiedenis.
Nee, helaas is Night at the Museum nog niet beschikbaar op Netflix in Nederland.
| Sound | Alan Silvestri | Original Music Composer |
| Production | Michael Barnathan | Producer |
| Writing | Robert Ben Garant | Screenplay |
| Art | Michele Laliberte | Art Direction |
| Art | Bridget McGuire | Art Direction |
| Camera | Guillermo Navarro | Director of Photography |
| Writing | Thomas Lennon | Screenplay |
| Directing | Shawn Levy | Director |
| Production | Shawn Levy | Producer |
| Production | Ilene Starger | Casting |
| Editing | Don Zimmerman | Editor |
| Art | Grant Van Der Slagt | Art Direction |
| Production | Chris Columbus | Producer |
| Art | Claude Paré | Production Design |
| Art | Lin MacDonald | Set Decoration |
| Art | Michael Diner | Art Direction |
| Visual Effects | Julie D'Antoni | Visual Effects Producer |
| Crew | J.J. Makaro | Stunt Coordinator |
| Art | Helen Jarvis | Supervising Art Director |
| Art | Chris Beach | Set Designer |
| Costume & Make-Up | Renée April | Costume Design |
| Visual Effects | Jim Rygiel | Visual Effects Supervisor |
| Production | Ellen Somers | Associate Producer |
| Production | Josh McLaglen | Co-Producer |
| Production | Thomas M. Hammel | Executive Producer |
| Production | Ira Shuman | Executive Producer |
| Production | Mark Radcliffe | Executive Producer |
| Production | Anne M. Simonet | Unit Production Manager |
| Production | Thomas M. Hammel | Unit Production Manager |
| Production | Ira Shuman | Unit Production Manager |
| Directing | Marty Eli Schwartz | First Assistant Director |
| Directing | Josh McLaglen | First Assistant Director |
| Directing | Misha Bukowski | Second Assistant Director |
| Crew | Blaise Corrigan | Stunt Driver |
| Directing | Brian Smrz | Second Unit Director |
| Art | Doug Girling | Set Designer |
| Art | Glenn Bydwell | Set Designer |
| Art | Peter Bodnarus | Set Designer |
| Directing | Daniel Mansfield | Second Second Assistant Director |
| Directing | Rhonda Taylor | Third Assistant Director |
| Directing | Diane Durant | Script Supervisor |
| Camera | David Crone | "A" Camera Operator |
| Sound | Michael Hibberson | Boom Operator |
| Camera | Glen A. Dickson | "B" Camera Operator |
| Camera | Scott Cozens | Camera Loader |
| Sound | Rob Young | Production Sound Mixer |
| Sound | John A. Larsen | Supervising Sound Editor |
| Sound | Craig Henighan | Supervising Sound Editor |
| Art | Ellen Freund | Property Master |
| Production | James Forsyth | Extras Casting |
| Art | Ray Lai | Graphic Designer |
| Art | Loree Cameron | Graphic Designer |
| Production | Zoe E. Rotter | Casting Associate |
| Production | Valerie Lindquist | Casting Assistant |
| Camera | James Salberg | Dolly Grip |
| Camera | Neil McBride | Dolly Grip |
| Costume & Make-Up | Blanche Boileau | Costume Supervisor |
| Costume & Make-Up | Karen Patch | Wardrobe Designer |
| Costume & Make-Up | Victoria Down | Makeup Artist |
| Costume & Make-Up | Sherry Linder-Gygli | Hairstylist |
| Production | Neil Robertson | Location Manager |
| Crew | William H. Orr | Special Effects Coordinator |
| Art | Jan Kobylka | Construction Coordinator |
| Art | John Dale | Construction Foreman |
| Crew | Gloria Davies | Unit Publicist |
| Crew | Rob Vreugde | Transportation Coordinator |
| Sound | Craig Henighan | Sound Designer |
| Sound | R.J. Kizer | ADR Supervisor |
| Sound | Mildred Iatrou | ADR Editor |
| Sound | Helen Luttrell | Dialogue Editor |
| Sound | Susan Dawes | Dialogue Editor |
| Sound | Dave Kulczycki | Sound Effects Editor |
| Sound | John Murray | Foley Supervisor |
| Sound | Donald Sylvester | Foley Editor |
| Sound | Warren Hendriks | First Assistant Sound Editor |
| Sound | Skip Longfellow | Assistant Sound Editor |
| Sound | Terry Wilson | Supervising Music Editor |
| Sound | Tim Gomillion | Sound Recordist |
| Sound | Dennis Rogers | Sound Recordist |
| Sound | Matt Patterson | Sound Recordist |
| Sound | Charleen Richards-Steeves | ADR Mixer |
| Sound | David Lucarelli | ADR Recordist |
| Sound | Dan O'Connell | Foley Artist |
| Sound | John T. Cucci | Foley Artist |
| Sound | James Ashwill | Foley Mixer |
| Editing | Gary Burritt | Negative Cutter |
| Editing | Chris Regan | Color Timer |
| Camera | Larry Blanford | Second Unit Director of Photography |
| Writing | Milan Trenc | Book |
| Crew | Kimberly Chiang | Stunts |
| Sound | Armen Ksajikian | Musician |
| Sound | Craig Ricci Shaynak | ADR & Dubbing |
| Sound | Stefán Karl Stefánsson | ADR & Dubbing |
| Crew | Lauro David Chartrand-DelValle | Stunts |
| Crew | Clint Carleton | Stunts |
| Editing | Dean Zimmerman | Associate Editor |
